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Canine interleukin 6 (IL-6) ELISA kit (15.6-1000 pg/mL)
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Cat. No.:
OB0125WXX-042
Synonyms:
IL-6 ELISA kit; Canine IL-6; Interleukin 6 (IL-6); Canine IL-6 ELISA kit
Size:
48T; 96T
Specification
Description:
Canine interleukin 6 (IL-6) ELISA kit (15.6-1000 pg/mL) is a ready-to-use microwell, strip plate ELISA kit for analyzing the presence of canine IL-6 in the range of 15.6-1000 pg/mL.
Background:
IL-6 is a cytokine with multiple biological functions. It is a potent inducer of the acute phase response, acting on T cells, B cells, hematopoietic progenitors, hepatocytes, and cells of the central nervous system. It plays an important role in the eventual differentiation of B cells into Ig-secreting cells. It also acts as an actin, which is excreted into the bloodstream after muscle contraction and can promote lipolysis and improve insulin resistance.
Formula Weight:
23,718 Da
Assay Principle:
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL-6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL-6). Next, avidin conjugated to HRP is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL-6), biotin-conjugated antibody, and enzyme-conjugated avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL-6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Type:
Double-antibody sandwich
Assay Target:
IL-6
Target Species:
Canine
Research Area:
Inflammatory research; Cytokine research; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
15.6-1,000 pg/mL
Sensitivity:
6.2 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Canine interleukin 6 (IL-6) ELISA kit (15.6-1000 pg/mL) is used to detect the level of canine IL-6 in the serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluid samples.
Storage:
Store at 2-8°C.