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Canine agouti-related protein (AGRP) ELISA kit (25-500 pg/mL)
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Cat. No.:
OB0525WXX-360
Synonyms:
Canine AGRP ELISA kit; Canine agouti-related protein (AGRP); Canine agouti-related protein; Agouti-related protein (AGRP); Canine AGRP; AGRP ELISA kit
Size:
48T; 96T
Specification
Description:
Canine agouti-related protein (AGRP) ELISA kit (25-500 pg/mL) is a ready-to-use ELISA kit for analyzing canine AGRP levels with a detection range of 25-500 pg/mL and a sensitivity of 1.0 pg/mL.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,440 Da
Assay Principle:
The AGRP ELISA Kit employs a competitive enzyme immunoassay technique utilizing a monoclonal anti-AGRP antibody and AGRP-HRP conjugate. The assay sample and buffer are incubated with the AGRP-HRP conjugate on a precoated plate for one hour. At the end of the incubation period, the wells are decanted and washed five times. The wells are then incubated with substrate for the HRP enzyme. The product of the reaction of the enzyme with the substrate forms a blue colored complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of AGRP because AGRP in the sample competes with the AGRP-HRP conjugate for the binding site of the anti-AGRP antibody. Since there are a limited number of binding sites, as more sites are occupied by AGRP in the sample, fewer sites remain to bind the AGRP-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of AGRP in each sample is derived from this standard curve.
ELISA Type:
Competitive
Assay Target:
AGRP
Target Species:
Canine
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Detection Range:
25-500 pg/mL
Sensitivity:
1.0 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Canine agouti-related protein (AGRP) ELISA kit (25-500 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in canine serum, plasma, cell culture supernatants, body fluid, and tissue homogenate samples.
Storage:
Store at 2-8°C.