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Canine adiponectin (ADP) ELISA kit (1.56-100 ng/mL, Up to 0.5 ng/mL)
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Cat. No.:
OB0525WXX-148
Synonyms:
Canine ADP ELISA kit; Adiponectin (ADP); Canine adiponectin; Canine adiponectin (ADP); ADP ELISA kit; Canine ADP
Size:
48T; 96T
Specification
Description:
Canine adiponectin (ADP) ELISA kit (1.56-100 ng/mL, up to 0.5 ng/mL) is a ready-to-use ELISA kit for analyzing canine ADP levels with a detection range of 1.56-100 ng/mL and a sensitivity of up to 0.5 ng/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The kit is based on the double antibody sandwich ELISA technique. The pre-coated antibody is anti-canine ADP monoclonal antibody, and the detection antibody is biotinylated polyclonal antibody. The samples and biotinylated antibodies are added into the wells of the ELISA plate separately, and then rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the wells. The enzyme conjugates are thoroughly washed with PBS or TBS and then colored with TMB substrate, which reacts with the peroxidase activity to produce a blue product, which finally turns yellow upon addition of the termination solution (color developing reagent C). The intensity of the color is positively correlated with the amount of target analyte in the sample.
ELISA Type:
Quantitative sandwich
Assay Target:
ADP
Target Species:
Canine
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Canine serum; Plasma; Cell culture supernatant
Detection Range:
1.56-100 ng/mL
Sensitivity:
Up to 0.5 ng/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Canine adiponectin (ADP) ELISA kit (1.56-100 ng/mL, Up to 0.5 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in canine serum, plasma, and cell culture supernatant samples.
Storage:
Store at 2-8°C.