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Bovine Interleukin 6 (IL-6) ELISA kit (5-1000 pg/mL)
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Cat. No.:
OB0125WXX-040
Synonyms:
IL-6 ELISA kit; Bovine IL-6; Interleukin 6 (IL-6); Bovine IL-6 ELISA kit
Size:
48T; 96T
Specification
Description:
Bovine Interleukin 6 (IL-6) ELISA kit (5-1000 pg/mL) is a ready-to-use microwell, strip plate ELISA kit for analyzing the presence of bovine IL-6 in the range of 5-1000 pg/mL.
Background:
IL-6 is a cytokine with multiple biological functions. It is a potent inducer of the acute phase response, acting on T cells, B cells, hematopoietic progenitors, hepatocytes, and cells of the central nervous system. It plays an important role in the eventual differentiation of B cells into Ig-secreting cells. It also acts as an actin, which is excreted into the bloodstream after muscle contraction and can promote lipolysis and improve insulin resistance.
Formula Weight:
23,759 Da
Assay Principle:
The microtiter plate provided in this kit has been pre-coated with goat anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IL-6 and Horseradish Peroxidase (HRP) conjugated IL-6. The competitive inhibition reaction is launched between HRP-labeled IL-6 and unlabeled IL-6 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-6 in the sample. The color development is stopped, and the intensity of the color is measured.
ELISA Type:
Quantitative competitive
Assay Target:
IL-6
Target Species:
Bovine
Research Area:
Inflammatory research; Cytokine research; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
5-1000 pg/mL
Sensitivity:
2.5 pg/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Bovine Interleukin 6 (IL-6) ELISA kit (5-1000 pg/mL) is used to measure bovine IL-6 in serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.