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Bovine agouti-related protein (AGRP) ELISA kit (78.0-5000 pg/mL)
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Cat. No.:
OB0525WXX-357
Synonyms:
Bovine AGRP ELISA kit; Bovine agouti-related protein (AGRP); Bovine agouti-related protein; Agouti-related protein (AGRP); Bovine AGRP; AGRP ELISA kit
Size:
48T; 96T
Specification
Description:
Bovine agouti-related protein (AGRP) ELISA kit (78.0-5000 pg/mL) is a ready-to-use microwell, strip, or full plate ELISA kit for analyzing bovine AGRP levels with a detection range of 78.0-5000 pg/mL.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,706 Da
Assay Principle:
The microtiter plate provided in this kit is pre-coated with an antibody specific for AGRP. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated polyclonal antibody preparation specific for AGRP, and HRP-conjugated avidin is added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing AGRP, biotin-conjugated antibody and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of AGRP in the sample can then be determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Quantitative ELISA
Assay Target:
AGRP
Target Species:
Bovine
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids.
Detection Range:
78.0-5000 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Bovine agouti-related protein (AGRP) ELISA kit (78.0-5000 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in bovine serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Storage:
Store at 2-8°C.