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Bovine adiponectin (ADP) ELISA kit (0.62-40.0 μg/mL)
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Cat. No.:
OB0525WXX-208
Synonyms:
Bovine ADP ELISA kit; Bovine adiponectin (ADP); Bovine adiponectin; Adiponectin (ADP); Bovine ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Bovine adiponectin (ADP) ELISA kit (0.62-40.0 μg/mL) is a ready-to-use ELISA kit for analyzing bovine ADP levels with a detection range of 0.62-40.0 μg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,133 Da
Assay Principle:
The microtiter plate provided in this kit is pre-coated with a specific ADP antibody. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated ADP-specific polyclonal antibody preparation, and HRP-conjugated avidin is added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.
Assay Target:
ADP
Target Species:
Bovine
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids
Detection Range:
0.62-40.0 μg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Bovine adiponectin (ADP) ELISA kit (0.62-40.0 μg/mL) is designed for the in vitro quantitative analysis of ADP levels in bovine serum, plasma, tissue homogenates, cell culture supernates, and other biological fluid samples.
Storage:
Store at 2-8°C.