Overview

Obesity is a complex, multifactorial disease involving an imbalance between energy intake and expenditure, leading to excessive lipid accumulation. This also leads to changes in the normal role of the liver in lipid and energy metabolism, which in turn causes a range of histopathologic changes. A detailed analysis of stem cell function is necessary for obesity research. Protheragen offers a one-stop shop for liver cell function analysis, based on extensive experience in Obesity Pathology Research. Our goal is to explore the mechanisms of obesity better and provide reliable data to support the development of anti-obesity therapies.

Unraveling the Mystery of Obesity - Precision Liver Cell Function Analysis Service

The primary objective of this service is to elucidate how obesity impacts liver cell function, encompassing aspects such as fat metabolism, glucose regulation, oxidative stress, and signaling pathways, as detailed below:

Fat Observation

Liver cells are observed to analyze the number, size, and distribution of fat droplets in order to assess the extent of hepatocellular steatosis. This process involves specific staining methods such as Sudan III staining or oil red O staining to facilitate the observation of fat droplets.

Liver Cell Gene Analysis and Signal Transduction Analysis

By employing state-of-the-art sequencing technology, we meticulously scrutinize the expression alterations of a multitude of genes within liver cells, unveiling the sophisticated molecular mechanisms that govern abnormal liver cell functionality. Moreover, we undertake an exhaustive examination of the activation states of signaling pathways pertinent to metabolic regulation and other critical biological processes within liver cells. Our objective is to acquire a comprehensive grasp of the profound implications that obesity has on liver cell signaling pathways.

Metabolomics Analysis

We evaluate the metabolic capacity of liver cells in processing carbohydrates, lipids, and other nutrients, as well as the consequences of these metabolic activities on energy generation and material deposition. Leveraging cutting-edge technologies, such as mass spectrometry, we undertake a systematic examination and analysis of the metabolite profiles within liver cell extracts, encompassing fatty acids, sugars, and bile acids. By elucidating the distinctive features of metabolic pathways in liver cells during obesity and exploring the correlations between alterations in key metabolites and obesity, we establish a scientific foundation for gaining profound insights into the mechanisms underlying obesity and for the formulation of effective intervention strategies.

Other Tests

In addition to this, we also conduct detailed studies on liver cell enzyme activities, oxidative stress, and so on. This includes measuring the level of oxidative stress markers in the liver cells, the activity of metabolism-related enzymes, the ability of the liver cells to take up and utilize glucose, etc.

One-stop Anti-obesity Research Support

Based on the detailed analysis of liver cell function, we also provide one-stop anti-obesity research services, which include the following:

Workflow

Liver cell functional analysis service involves obtaining liver cell samples from cell culture or animal models. The metabolism, signaling, and other functions of the liver cells are then comprehensively assessed by biochemical, molecular, and cellular biological techniques (e.g. enzyme activity assay, protein blotting, real-time quantitative PCR (qPCR), flow cytometry, and high-throughput sequencing). After collecting and analyzing the data, we understand the physiological and pathological mechanisms of liver cells, which provide reliable support for new drug discovery and development, disease modeling, and optimization of therapeutic strategies.

Applications

• By assessing liver cell function, researchers can gain insight into the mechanisms that link obesity to abnormal liver function.
• Understanding these liver cell-specific changes provides important insights for the development of strategies to treat obesity and its complications, including liver disease and type 2 diabetes.
• During drug development, it can help predict the hepatotoxicity and safety of drugs by assessing the effects of new drugs on liver cell function.

Advantages

• Our liver cell function analysis service provides end-to-end research support, from protocol design to data interpretation.
• Based on the support of a dedicated research team, we provide customized liver cell function study designs, high-quality laboratory bureaus, and reliable biological insights.
• Our analysis of liver cell metabolites covers a wide range of metabolites to support in-depth insights.

Publication Data

Frequently Asked Questions

1. What types of analyses are included in the liver cell functional analysis service?

   Liver cell functional analysis includes assessment of metabolic pathways, lipid accumulation, gene expression profiling, enzyme activity analysis, etc., in liver cells. Depending on the content of the analysis, the time required for the entire service will vary. After understanding the client's specific experimental needs, we customize the relevant analysis journey and complete the relevant experiments efficiently.

2. What techniques are used in liver cell function analysis?

   Liver cell functional analysis involves a variety of experiments and assays, including enzyme activity assessment, lipid accumulation measurements, metabolite assays, transcriptomic and proteomic analyses, and more. Techniques involved include imaging techniques, mass spectrometry, flow cytometry, qPCR, Western blotting, and more. Through comprehensive analysis, we understand the changes in liver cell function in the obese state and provide useful information for in-depth investigation of obesity mechanisms and therapy development.

At Protheragen, our liver cell function analysis services are professional, comprehensive, customized, and efficient, providing strong support to researchers. We not only help to gain a deeper understanding of the impact of obesity on liver function and its mechanisms but also contribute to advancing research on the prevention and treatment of obesity-related diseases. Please do not hesitate to contact us if you have any analysis needs.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Adipose Tissue Cell Function Analysis Service