Overview

Obesity is a chronic metabolic disease that is not only related to dietary habits but is also influenced by the inter-regulation between environmental, behavioral, and genetic factors. Genome-wide association studies have shown that a variety of genes are associated with obesity, such as genes related to glucose homeostasis, metabolism, or obesity phenotype. Editing or modulating the expression of these genes in animal models helps to gain insight into the genetic mechanisms of obesity and develop promising strategies for treating obesity. Protheragen offers a custom single-gene editing model service combining multiple precision gene editing technologies. We have a deep understanding of obesity and provide support and insights for a variety of related studies.

High-quality Single Gene Editing Models Through Superb Technical

Combining comprehensive assay technologies and gene editing tools, we realize custom single gene editing model service from protocol design to model validation. The specific construction process includes the following:

• Construction of Obesity Model

  Gene-edited animal models for obesity research mainly include Mice, Rats, Zebrafish, Rabbits, and so on. We utilize various gene editing tools to efficiently construct various gene-modified animal models. By regulating genes of interest related to obesity, we delve into the relationship between these genes and obesity, specific anti-obesity therapies targeting these genes, and others. The types of customized single gene editing models include the following:

  • Customized single gene knock-in models: We insert a variety of exogenous genes of interest into the target locus to understand gene function in more detail and to determine the impact of functional changes. The entire process is simple and the model highly mimics the target characteristics. These single knock-in models have a wide range of applications for gene function, obesity research, therapy development, and more.
  • Customized single-gene knockout models: We knock out various obesity-related genes, such as the Leptin gene, Uncoupling Protein-1 (UCP1) gene, etc., and detect the associated physiological changes. These knockout models not only provide information on the regulation of food intake and energy balance but also provide useful tools for validating innovative anti-obesity therapies and screening anti-obesity drugs against specific targets.

  We have a variety of gene editing techniques to achieve specific gene knockout or knock-in modifications in animal models. Specific modifications to these genes are used to understand their function and facilitate the development of novel anti-obesity therapies.

  • Gene targeting: It is essentially the replacement of endogenous genes by homologous recombination. Genetic modification through gene targeting is one of the most common tools used to study the genetic mechanisms of obesity.
  • Other gene editing techniques: We have a wide range of gene manipulation efforts to achieve controlled genomic modifications efficiently and rapidly. Using these precise gene editing techniques to realize efficient gene knock-in, knock-out, precise point editing, and single base editing, we have constructed a variety of obesity models, including rats, mice, rabbits, and so on.

• Validation of Single Gene Editing Models

  The accuracy of the single gene editing model is validated by sequencing, pathological characterization, and biomarker analysis. Parameters tested include body weight, adipose tissue, blood biochemical analysis, insulin tolerance test, glucose tolerance test, etc. Based on the results, we further optimize the modeling process to improve reproducibility and accuracy.

Workflow

Applications

• Single-gene editing models are useful tools for screening obesity-related genes, more comprehensively studying the relationship between certain genes and obesity, and the genetic mechanisms of obesity.
• Single-gene editing models are used to develop and screen various anti-obesity drugs against specific targets and to study their therapeutic and toxicological effects.
• Single gene editing models are useful tools to assess the efficacy and safety of anti-obesity gene therapies.

Advantages

• With rich experience in single gene editing model construction, our experienced scientists customize the most suitable experimental protocols for our clients' research purposes.
• We use various tools to construct single gene editing models with high precision of gene editing.
• We always put our clients first, continuously answer questions, and provide excellent after-sales service.

Personalized Services and Highly Flexible Solutions

We are committed to building highly flexible and customized solutions to support our clients at every step of their research journey, ensuring that they achieve breakthroughs at every critical research juncture. In addition to the construction of obesity models, we also provide a full range of obesity risk assessment, diagnostic testing, obesity prediction, and gene editing services. In addition, biomarker, microbiome, and pathology analyses are also essential in obesity research. Our team consists of distinguished and highly qualified scientists. They tailor-make innovative assays based on a deep understanding of our clients' experimental needs. At the same time, as the most reliable partner, we also provide customized solutions for obesity and weight management.

Publication Data

Frequently Asked Questions

What metrics are tested for validation of single gene editing models?

Testing parameters vary depending on the type of model. Typical parameters include body weight, number of obese animals, food intake, behavior, glucose tolerance, insulin tolerance, adipocyte size, liver tissue, and blood biochemistry.

How long does it usually take to build a single gene editing model?

The length of model construction varies depending on the specific study objectives and experimental requirements and typically ranges from a few weeks to a few months. After understanding your needs, we will develop a detailed experimental process and timeline and then communicate with you.

Protheragen is a professional supplier of gene editing and obesity animal model construction, providing high-quality customized single-gene editing model service. We offer a wide range of animal models for basic obesity research, the development of anti-obesity drugs, and anti-obesity therapies targeting specific loci. Please feel free to contact us to learn more about custom single gene editing model services if you need them in this area. We look forward to supporting your research with our rich experience and excellent experimental techniques.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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