Overview

Obesity is a chronic metabolic disease and is regulated by complex interactions between genetic, behavioral, and environmental factors. Understanding the mechanism of action of each gene type in obesity helps to understand better the causes of obesity and design targeted obesity treatment and intervention strategies.

Targeted Gene Editing for Obesity Solutions

Protheragen provides a one-stop obesity single gene editing service, including screening and identification of target genes, gene editing, validation, and detailed analysis of results. Our professional service team ensures the high-quality completion of each project, providing high-quality research tools for Obesity Research and strong support for pharmaceutical development.

Selection and Identification of Target Genes

We screen and identify genes that are directly related to obesity, such as genes related to fat metabolism, energy expenditure, and appetite regulation. After that, we select the appropriate editing method according to the characteristics of the target genes and editing needs, and confirm the editing sites, such as knockout, replacement, or insertion.

Gene Editing

We culture the target cells (e.g., adipocytes, stem cells, etc.) and apply gene editing technology to edit them. The effect of gene editing is verified by sequencing, PCR, and other methods to determine whether the gene is edited successfully. Animal gene editing model construction also involves screening, mating, and obtaining stable lines.

Validation

We use RT-PCR, western blot, etc., to analyze the gene expression level, each metabolic parameter, behavior, body weight, and body fat content, and others in order to analyze the phenotypic changes after gene editing.

Workflow

Applications

• The obesity single gene editing service is suitable for evaluating the roles of key genes and their products in metabolism, adipose tissue development, and the pathobiology of obesity.
• Using single-gene editing technology, obesity models can be constructed, and the pathological process of obesity can be simulated in the models, providing an important analytical tool for the study of the pathogenesis of obesity and preventive measures.
• Obesity single gene editing services are suitable for constructing specific gene editing models and laying the foundation for effectively evaluating the effects of anti-obesity drugs.

Advantages

• Our gene editing experts have been deeply engaged in gene editing for many years, with rich theoretical knowledge and project experience, and provide the best solutions.
• We provide comprehensive gene editing technology services, including single gene knockout, point mutation, specific gene knock-in, etc., and can complete customized solutions based on client needs.
• Based on advanced research equipment and the rich experience of our technicians, we can realize accurate gene editing operations in a short period of time and a short project cycle.

One-stop Anti-obesity Research Support

Based on the highly efficient gene editing technology, we also provide the following services to support our clients' research on anti-obesity therapies.

Publication Data

Frequently Asked Questions

1. What data can single-gene editing provide for obesity research?

   Single gene editing can help provide insights into the functions of various obesity potential genes in metabolism, adipogenesis, etc., their interactions with environmental factors, and the biological pathways involved in obesity, leading to a more comprehensive understanding of obesity. Gene therapy has great potential in the fight against obesity. Single-gene editing also provides an opportunity to explore the role of gene therapy in the treatment of obesity and related complications.

2. How do you verify that gene editing is successful?

   We validate it through a variety of methods, including PCR amplification and sequencing, and gene expression level detection. Our researchers analyze the success of gene validation by analyzing whether the intended editing of the target gene sequence has occurred, the efficiency and specificity of the editing, and whether there are changes in gene expression.

Protheragen has a highly theoretical and practical R&D team that is capable of utilizing cutting-edge gene editing methods to achieve precise and efficient editing of individual genes. Please contact us for professional bioanalytical and gene editing services to safeguard your research progress and results.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Candidate Obesity Gene Screening Service
• Obesity Gene Knockin Service
• Obesity Gene Knockout Service
• Obesity Gene Overexpression Service
• Obesity Gene Silencing Service
• Obesity Multigene Gene Editing Service