Explore Obesity Mechanism, Lead Scientific Research Innovation - Obesity Multigene Editing Service

Advances in understanding the molecular basis of obesity and obesity-related diseases are critical to the development of novel therapeutic strategies and preventive approaches. Protheragen offers a full range of Obesity Research Services and utilizes a variety of advanced gene editing technologies to enable Targeted Editing of Obesity-related Genes. In order to meet the needs of researchers in exploring obesity mechanisms and developing therapies, we have launched the obesity multigene editing service. This service combines advanced gene editing technology and comprehensive research support, aiming to help scientists reveal the complex mechanisms of obesity and promote cutting-edge advances in obesity.

We utilize cutting-edge gene editing tools to accurately edit obesity-related genes, providing researchers with one-stop solutions from gene screening, protocol design, implementation, to effect verification. Through editing, we efficiently construct obesity-related gene knockout, knock-in, or point mutation models, providing reliable tools for obesity research.

Gene Screening and Identification

Obesity is linked to a diverse range of genes, encompassing those that code for proteins/enzymes involved in food consumption, the production of fats (lipogenesis), glucose metabolism, the breakdown of fats (lipolysis), and the storage of fats in adipose tissue. Notable examples include leptin, a crucial regulator of energy intake that impacts appetite and hunger sensations. Additionally, genes that encode uncoupling proteins (UCPs) are also implicated with obesity, as these proteins are responsible for non-shivering thermogenesis, a process predominantly occurring in brown adipose tissue. Our researchers also utilize genomics and other technical tools to screen for genes associated with obesity and use gene editing to validate their function.

Gene Editing

We select appropriate gene editing tools, construct appropriate vectors, and perform Knockout and Knock-in operations on specific cells through adeno-associated virus (AAV) transfection, electroporation, and other techniques to achieve targeted modification of genetic information. Afterwards, positive clones are obtained through screening and sequenced to test whether the editing is successful.

Validation

We culture the gene-edited cells and use molecular biology, cell biology, and other technical means to detect the phenotypic and functional changes after editing. In addition, we also conduct detailed validation of the gene editing model, including sequencing, phenotypic analysis of the animal model, physiological function measurement, and potential genetic stability, etc., to ensure that the established model can accurately simulate the target state and provide a reliable basis for subsequent obesity research.

Workflow

We provide a one-stop obesity multi-gene editing service. First, we identify obesity-related target genes and their functions, and design and synthesize editing systems for these genes. Afterwards, we introduce the editing system into the target cells, verify whether the gene editing is successful by PCR, sequencing, and other technologies, and then conduct cell phenotype and function analysis to evaluate the editing effect.

Applications

• Basic research on obesity: Through multi-gene editing technology, multiple genes related to obesity can be precisely altered to study the specific role of these genes in the occurrence and development of obesity.
• Research on novel therapeutic strategies: Based on multi-gene editing technology, more effective gene therapies for obesity can be developed by precisely editing obesity-related genes.
• Drug screening and evaluation: Obesity-related gene editing models constructed using multi-gene editing technology can be used for drug screening and evaluation. This helps to identify new drugs with potential therapeutic effects and evaluate their safety and efficacy.

Advantages

• We provide personalized gene editing solutions to meet diverse research needs.
• We use advanced gene editing tools and technologies to ensure high editing efficiency and specificity of obesity-related genes.
• We have an interdisciplinary team of researchers with extensive experience in gene editing, biochemical research, and bioinformatics analysis to provide comprehensive technical support and guidance.

One-stop Anti-obesity Research Service

Based on good editing technology, we also provide good anti-obesity research services, including target screening, anti-obesity therapeutic development, and preclinical research of therapies.

Publication Data

Frequently Asked Questions

1. What species is the obesity multigene editing service available for?

   This service is available for a wide range of sample types, including various cellular and animal models (mice, rats, etc.). Through gene editing technology, we create specific obesity phenotypes to study the function of obesity-related genes, the physiopathological mechanisms of obesity, and potential therapeutic approaches.

2. How to validate the obesity multigene editing results?

   The multi-gene editing validation process involves multiple steps, including verification of the efficiency and site specificity of gene editing by sequencing, and detection of phenotypic and functional changes in the edited cells using functional assays and other methods. Western blot, metabolomics analysis, etc., are also utilized to comprehensively analyze the effects of gene editing on phenotype.

Protheragen has developed a one-stop obesity multi-gene editing service and provides reliable and extensive support for all aspects of obesity research. If you have any questions or need further information about the obesity multigene editing service, please feel free to contact us. We will be happy to provide you with professional answers and personalized service solutions.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Candidate Obesity Gene Screening Service
• Obesity Gene Knockin Service
• Obesity Gene Knockout Service
• Obesity Gene Overexpression Service
• Obesity Gene Silencing Service
• Obesity Single Gene Editing Service