Obesity is a complex metabolic disease involving the interaction of numerous genes. In-depth understanding and precise regulation of the functions of these genes is the key to unraveling the mechanisms of obesity and related metabolic diseases and to developing efficient therapies.

Precision Gene Silencing for Obesity Research Discovery

Protheragen offers a one-stop obesity gene silencing service based on its deep expertise in molecular biology and Obesity Research. We provide a full range of Gene Editing solutions from gene target selection, silencing vector design and construction to in vitro cellular analysis and in vivo animal model validation, helping you to precisely study the function of specific genes and accelerate your research process.

Project Consultation and Program Design

We have in-depth communication with the client and discuss the research objectives, gene targets of interest, expected research models, and other relevant information. We then customize the optimal gene silencing strategy and delivery method (electroporation, plasmid transfection, virus infection, etc.).

Construction and Preparation of Gene Silencing Vectors/Molecules

• siRNA synthesis and optimization: According to the target gene sequence, we design and synthesize highly efficient and specific siRNA sequences.
• shRNA vector construction and viral packaging: We design shRNA sequences for target genes and clone them into viral expression vectors.
• In addition, other gene silencing strategies are also available.

Transformation

• Cell models: We customize specific gene-silencing cell lines as needed. Silencing molecules are introduced into the cells using transfection reagents and viral vectors. The cell lines are then amplified and screened for stable silencing.
• Animal models: We customize a variety of gene-silencing animal models (nematode, mouse, rat, etc.). We deliver viral vectors or siRNAs to animals via lipid nanoparticles, injections, etc., and screen them for mutations.

Verification of Silencing Efficiency

We analyze the silencing efficiency by detecting changes in mRNA expression levels and related protein expression levels through reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot and other technologies.

Functional Assay

We perform detailed tests on lipid metabolism, secretion of relevant inflammatory factors, adipogenesis, histopathology, body weight, and safety to analyze the relevant changes caused by the silencing of specific genes.

Analysis Report

In the end, we provide a detailed experimental report, including the experimental protocol, raw data, analysis results, pictures, conclusions, and other detailed information.

Workflow

Applications

• Gene expression can be regulated by gene silencing, and the function of the gene in metabolism, capacity regulation, insulin resistance, etc., and its effect on related signaling pathways can be deeply analyzed.
• In vitro or in vivo transgenic models of specific gene functions can be constructed through gene silencing for the study of obesity and related complications.
• Drug screening or efficacy evaluation on constructed gene-silencing cell or animal models can help promote the process of anti-obesity drug development.

Advantages

• We understand the complexity of obesity development and have rich experience in obesity-related genes and signaling pathways.
• We provide end-to-end solutions from gene target identification to in vivo functional validation to accelerate the research process.
• We conduct stringent quality control on vector construction, cell transfection, animal modeling, gene silencing efficiency, and downstream functional assays to ensure reliable data.

One-stop Anti-obesity Research Support

We also provide one-stop anti-obesity research services, which include the following:

Publication Data

Frequently Asked Questions

1. Can you silence any gene?

   Theoretically, we design gene silencing programs as long as the gene sequence is known. However, for some genes (e.g., highly conserved, lowly expressed, or with complex spliceosomes), silencing efficiency may be challenging. We will conduct a pre-feasibility assessment.

2. What are the main gene silencing techniques you use? What are their differences?

   We use siRNA, shRNA, and other editing technologies. siRNA is suitable for transient silencing and is easy to manipulate. shRNA is delivered via viral vectors for stable, long-term gene silencing and is suitable for constructing stable cell lines or in vivo experiments.

Protheragen recognizes that every research project is unique and offers flexible service options to precisely match your research needs. During the course of your project, our team is always available to provide professional technical advice and answers to questions, and we are committed to keeping all of our clients' project information and data strictly confidential. Please feel free to contact us for more information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Candidate Obesity Gene Screening Service
• Obesity Gene Knockin Service
• Obesity Gene Knockout Service
• Obesity Gene Overexpression Service
• Obesity Multigene Gene Editing Service
• Obesity Single Gene Editing Service