Obesity, as a global health challenge, has a complex developmental mechanism involving genetic, environmental, and behavioral factors. Understanding the function of specific genes in the development of obesity and related metabolic diseases is critical to unraveling the pathogenic mechanisms, identifying potential therapeutic targets, and facilitating the development of related therapies.

Build Obesity Gene Overexpression Models, Accelerate Mechanism Research

Protheragen specializes in the field of obesity research and has built an advanced molecular biology technology platform. We understand the critical role of genes in the development of obesity and provide high-quality obesity gene overexpression services. Based on our rich project experience, we provide precise and personalized technical support to various researchers and contribute to the study of obesity mechanisms and therapies.

Target Gene Identification and Overexpression Vector Construction

After identifying the obesity-related target genes of interest, we design the relevant experimental procedures based on the gene sequence information provided by the client. Based on the mature and stable gene interference expression system, we construct expression vector systems (lentiviral vectors, adenoviral-associated vectors, etc.).

We also provide large-scale preparation and purification of vector plasmids to ensure vector quality and yield.

Cell Transfection

We optimize transfection or transduction conditions for different cell types and vector systems to improve gene delivery efficiency. Through screening (e.g., antibiotic screening), we construct cell lines with stable overexpression of target genes to provide stable models for long-term research.

Overexpression Efficiency Assay

We evaluate the expression level of target genes by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot, immunohistochemistry, and other methods. Relevant sterility tests (bioburden test, mycoplasma test, etc.) are also carried out. Testing methods is selected according to actual requirements.

Gene Overexpression in Animal Models

Relevant vectors are constructed and injected into specific sites by microinjection and other means. After that, the transgenic animals are screened, and the stable system is obtained by mating.

Downstream Functional Verification and Analysis

For cell-stable transfected lines, we perform various types of phenotypic analysis and functional validation, including cell viability, cytotoxicity, cell proliferation, migration, apoptosis, and so on.

For animals, we perform detailed analysis of phenotypes, including body weight, body fat content, food intake, energy expenditure, glucose tolerance, insulin sensitivity, inflammatory factor detection, etc.

Workflow

Applications

• Gene overexpression under different nutritional or exercise conditions is used to study and validate gene regulatory functions and roles in obesity-related processes.
• Constructed cellular or animal models of target gene overexpression mimic the process of obesity and provide models for mechanistic studies and anti-obesity drug screening.
• Constructed cell or animal models overexpressing target genes are used to evaluate various anti-obesity therapies and analyze their safety.

Advantages

• Based on advanced gene editing technology, dozens or hundreds of times overexpression levels can be realized, and the expression of target genes is significantly elevated after regulation.
• Based on years of experience in molecular biology experiments, we have a rich variety of screening labels to facilitate all kinds of experiments.
• We provide flexible and diversified customized solutions and prefer different experimental systems for different genes and cell characteristics to achieve the best regulatory effect.

One-stop Anti-obesity Research Support

Publication Data

Frequently Asked Questions

1. What information do I need to provide to start a project?

   You need to provide information about the target gene of interest (gene name, accession number, etc.), the research objectives, the intended experimental model (cell line or animal model), and any specific experimental needs.

2. How to evaluate gene overexpression efficiency?

   We usually analyze gene overexpression efficiency by detecting the expression of genes by RT-qPCR and the expression of related proteins by western blot, etc.

3. Can I choose only some of the services?

   Of course, you can choose part of the service according to your research needs, such as vector construction only or selecting specific QC validation experiments.

Protheragen provides efficient, reliable, and highly customized solutions to facilitate easier verification of target gene function and accelerate the obesity research process. If you are interested in our obesity gene overexpression service or have any questions, please do not hesitate to contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Candidate Obesity Gene Screening Service
• Obesity Gene Knockin Service
• Obesity Gene Knockout Service
• Obesity Gene Silencing Service
• Obesity Multigene Gene Editing Service
• Obesity Single Gene Editing Service