Precision Gene Knockout: Unlocking Obesity Secrets

A precise understanding of the function of specific genes plays a crucial role in the study of obesity and related complications. Protheragen provides a high-quality obesity gene knockout service with in-depth expertise and cutting-edge Gene Editing technology. We are committed to ensuring that you obtain the ideal knockout model through efficient gene editing and one-stop solutions to accelerate obesity mechanism research, target validation, and innovative therapy development.

Target Gene Analysis and Evaluation

Our expert team conducts in-depth bioinformatics analysis based on target gene information and research objectives to assess the potential role of genes in obesity development, including the expression, function, and correlation between target genes and obesity.

Knockdown Strategy Design

Based on the characteristics of the target gene and the experimental requirements, we design the optimal knockout strategy and adopt efficient gene editing strategies to realize the specific knockout of the gene.

Model Construction

The gene knockout service applies to cell and animal models. We construct relevant vectors and introduce them into suitable cells using efficient transfection methods. Afterwards, we analyze whether the target vector is precisely integrated into the target gene locus through screening and characterization. Finally, the knockout cell lines or animals are obtained through validation.

Genetic Analysis and Functional Validation

DNA extraction is performed on the obtained cells or animal models, and the knockout efficiency and editing sites of the target genes are verified by PCR and sequencing to ensure the specificity of genome editing.

Perform detailed phenotypic analysis of the knockout model to study the effects of the knockout on its physiology, biochemistry, and behavior. Phenotyping analysis is customized upon request.

Data Analysis and Reporting

Upon completion of the project, we also deliver detailed experimental data analysis reports, including genotype identification results, gene/protein expression validation results, and phenotypic data analysis. We also provide professional consultation and assistance for any problems encountered during the follow-up research.

Workflow

Applications

• Knockout allows in-depth investigation of the gene's role in obesity-related processes such as adipocyte differentiation, metabolism, energy expenditure, and appetite regulation.
• Knockout models are used to develop and validate potential drug targets for obesity treatment.
• Relevant models constructed by gene knockout are used to evaluate various anti-obesity therapies and play a crucial role in optimizing these therapies.

Advantages

• We have been deeply involved in the field of obesity for many years, have a deep understanding of obesity-related genes, pathways, and research models, and provide services precisely according to the different requirements of our clients.
• We provide end-to-end services, including comprehensive technical support and after-sales service from experiment design to data analysis.
• We master a variety of cutting-edge gene editing technologies and optimize the experimental process to improve knockdown efficiency and specificity.

One-stop Anti-obesity Research Support

Publication Data

Frequently Asked Questions

1. Are the knockout cell lines you deliver pure or heterozygous?

   Typically, we screen and deliver purebred clones to ensure complete inactivation of gene function.

2. How much does the knockout service cost?

   The price of the service depends on the complexity of the project, the characteristics of the target gene, the type of model (cell line or animal model), the knockout strategy, and the specified phenotypic analysis. After understanding the specific needs of the client, we provide a detailed project proposal and quotation.

Protheragen is committed to assisting obesity-related gene research with precise gene editing technology. Please feel free to contact us with your project requirements and target gene information. Our technical experts communicate with you promptly to understand your research objectives and customize the most appropriate experimental plan for you.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Candidate Obesity Gene Screening Service
• Obesity Gene Knockin Service
• Obesity Gene Overexpression Service
• Obesity Gene Silencing Service
• Obesity Multigene Gene Editing Service
• Obesity Single Gene Editing Service