Overview

Nematode, as a non-mammal obesity model, has become an important In Vivo Experimental Model in life sciences, due to the advantages of small size, short life cycle, rapid reproduction, easy handling, complete genetic information, highly conserved genes with human diseases, and convenient gene manipulation. Protheragen provides preclinical studies of anti-obesity therapeutics to clients worldwide.

Exploring the Story of Obesity with Our Nematode Model

• Nematode Obesity Model Generation

  Diet-induced obesity: Our company focuses on inducing nematode obesity through high-cholesterol diets and high-sugar diets. Our researchers have induced the generation of nematode obesity models by adding cholesterol or sugar to the nematode culture medium.

  Gene engineering: Nematodes are easily manipulated by genetic tools. Our researchers use gene editing and genome-scale RNA interference (RNAi) to modify specific genes to model nematode obesity. For example, nematodes with mutations in the daf-2 gene have increased fat accumulation and promote glycogen production. daf-16 gene is at the center of the regulation of multiple signaling pathways, is regulated by a variety of pathways, and has a regulatory role in nematode fat deposition.

• Nematode Obesity Model Analysis

  Measurement of nematode physiological indices

  • Longevity
  • Pharyngeal pumping rate

  • Number of offspring
  • Heat stress resistance

  • Athletic ability
  • Resistance to oxidative stress

  Determination of nematode biochemical indicators

  • Lipofuscin
  • Enzyme activity

  • Reactive oxygen species
  • Malondialdehyde (MDA)

  • Oil red staining
  • Triglyceride (TG)

  Special analysis

  • Fluorescent protein
  • Fat deposition

  • mRNA expression
  • Fat synthesis pathway

  • Metabolites
  • Fat catabolic pathway

Workflow

The nematode obesity model construction process usually involves the steps of selecting target nematode species, designing experimental protocols, designing induction protocols, screening and validating the model, and finally performing phenotypic analysis and functional validation.

Applications

• The nematode obesity model can be used to study genetic and environmental factors in obesity and provide a theoretical basis for preclinical studies.
• The nematode obesity model can be used in many research areas to perform rapid, large-scale, high-throughput screening using genetic modifications to discover drugs and bioactive compounds.
• An in-depth study of the metabolic mechanisms of nematode obesity models helps reveal the pathogenesis and treatment of metabolic diseases such as obesity.
• The nematode obesity model can be used to analyze the role of fatty acid synthesis, glycolysis, and other metabolic pathways in obesity, providing a basis for drug development.

Advantages of Us

• Relying on advanced genetics techniques, our researchers provide identification and analysis of obesity-related genes in nematode obesity models.
• Our company supports the integration of nematode obesity models with other biological models to expand their potential in drug screening and metabolic disease research.
• Our researchers have proven experience in genetic engineering experiments to ensure accurate operations.

Unlocking the Molecular Secrets of Obesity with Protheragen

As a leading biotechnology company, we have extensive experience and exceptional expertise in anti-obesity research. Our team is well versed in anti-obesity target discovery, development of reliable therapies, and critical preclinical studies. By choosing us, you will gain a trusted partner. We promise to be your best helper in anti-obesity research with efficient solutions.

Publication Data

Frequently Asked Questions

What are the enzymes or proteins involved in the lipolysis pathway in nematodes? 

The pathway of fatty acid breakdown in nematodes is similar to that in humans. Enzymes or proteins involved in the lipolysis pathway in nematodes include fatty acid translocase (FAT/CD36), fatty acid transfer protein (FATP), fatty acid binding protein (FABP), acyl-coenzyme A binding protein (ACBP), carnitine-palmitoyltransferases (CPTs), and ATP-binding cassette (ABC) transfer protein.

What are the core pathways involved in the regulation of nematode lipid metabolism?

The core pathways include insulin and transforming growth factor beta (TGFB) signaling pathways, nuclear hormone receptor-mediated signaling pathways, signaling pathways regulated by the transcriptional regulator sterol regulatory element-binding protein, and 5-hydroxytryptamine-mediated signaling pathways.

Protheragen is an industry-leading preclinical anti-obesity therapy research company dedicated to providing our clients with high-quality nematode obesity models. Our experienced research team is your most reliable obesity modeling partner. Please feel free to contact us at your convenience.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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